Journal: Differentiation; research in biological diversity
Article Title: LNGFR + THY-1 + human pluripotent stem cell-derived neural crest-like cells have the potential to develop into mesenchymal stem cells.
doi: 10.1016/j.diff.2016.04.003
Figure Lengend Snippet: Fig. 3. Cell type-specific marker analysis of human iPSC-derived LNGFRþTHY-1þ cells. (A, B) Human iPSC (WD39) derived LNGFRþTHY-1þ cells expressed NCC markers (AP2α, SOX10, NESTIN and HNK-1) as determined by immunocytochemistry (A) and by flow cytometry (B). (C) LNGFRþTHY-1þ cells were negative for the pluripotent stem cell markers, NANOG and OCT3/4, by immunocytochemical analysis. (D) LNGFRþTHY-1þ cells expressed neither hematopoietic cell markers nor the epithelial cell surface marker (CD326) by flow cytometric analysis. Scale Bars: 50 mm.
Article Snippet: After one hour incubation with blocking buffer (Nacalai Tesque) at room temperature, the following primary antibodies were applied: mouse monoclonal anti-human Ki-67 (MM1; Leica, 1:200), goat polyclonal anti-human SOX10 (R&D Systems, 1:200), rabbit monoclonal anti-human AP2α (C83E10; Cell Signaling Technology, 1:100), rabbit polyclonal anti-human NESTIN (Eurofins Genomics, 1:200), rabbit polyclonal anti-human NANOG (ReproCELL, 1:200), and mouse monoclonal anti-human OCT3/4 (C-10; Santa Cruz, 1:200).
Techniques: Marker, Derivative Assay, Immunocytochemistry, Cytometry