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rabbit anti ap2α  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti ap2α
    Rabbit Anti Ap2α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ap2α/product/Cell Signaling Technology Inc
    Average 94 stars, based on 42 article reviews
    rabbit anti ap2α - by Bioz Stars, 2026-03
    94/100 stars

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    Fig. 3. Cell type-specific marker analysis of human iPSC-derived LNGFRþTHY-1þ cells. (A, B) Human iPSC (WD39) derived LNGFRþTHY-1þ cells expressed NCC markers <t>(AP2α,</t> SOX10, NESTIN and HNK-1) as determined by immunocytochemistry (A) and by flow cytometry (B). (C) LNGFRþTHY-1þ cells were negative for the pluripotent stem cell markers, NANOG and OCT3/4, by immunocytochemical analysis. (D) LNGFRþTHY-1þ cells expressed neither hematopoietic cell markers nor the epithelial cell surface marker (CD326) by flow cytometric analysis. Scale Bars: 50 mm.
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    Fig. 3. Cell type-specific marker analysis of human iPSC-derived LNGFRþTHY-1þ cells. (A, B) Human iPSC (WD39) derived LNGFRþTHY-1þ cells expressed NCC markers <t>(AP2α,</t> SOX10, NESTIN and HNK-1) as determined by immunocytochemistry (A) and by flow cytometry (B). (C) LNGFRþTHY-1þ cells were negative for the pluripotent stem cell markers, NANOG and OCT3/4, by immunocytochemical analysis. (D) LNGFRþTHY-1þ cells expressed neither hematopoietic cell markers nor the epithelial cell surface marker (CD326) by flow cytometric analysis. Scale Bars: 50 mm.
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    Fig. 3. Cell type-specific marker analysis of human iPSC-derived LNGFRþTHY-1þ cells. (A, B) Human iPSC (WD39) derived LNGFRþTHY-1þ cells expressed NCC markers (AP2α, SOX10, NESTIN and HNK-1) as determined by immunocytochemistry (A) and by flow cytometry (B). (C) LNGFRþTHY-1þ cells were negative for the pluripotent stem cell markers, NANOG and OCT3/4, by immunocytochemical analysis. (D) LNGFRþTHY-1þ cells expressed neither hematopoietic cell markers nor the epithelial cell surface marker (CD326) by flow cytometric analysis. Scale Bars: 50 mm.

    Journal: Differentiation; research in biological diversity

    Article Title: LNGFR + THY-1 + human pluripotent stem cell-derived neural crest-like cells have the potential to develop into mesenchymal stem cells.

    doi: 10.1016/j.diff.2016.04.003

    Figure Lengend Snippet: Fig. 3. Cell type-specific marker analysis of human iPSC-derived LNGFRþTHY-1þ cells. (A, B) Human iPSC (WD39) derived LNGFRþTHY-1þ cells expressed NCC markers (AP2α, SOX10, NESTIN and HNK-1) as determined by immunocytochemistry (A) and by flow cytometry (B). (C) LNGFRþTHY-1þ cells were negative for the pluripotent stem cell markers, NANOG and OCT3/4, by immunocytochemical analysis. (D) LNGFRþTHY-1þ cells expressed neither hematopoietic cell markers nor the epithelial cell surface marker (CD326) by flow cytometric analysis. Scale Bars: 50 mm.

    Article Snippet: After one hour incubation with blocking buffer (Nacalai Tesque) at room temperature, the following primary antibodies were applied: mouse monoclonal anti-human Ki-67 (MM1; Leica, 1:200), goat polyclonal anti-human SOX10 (R&D Systems, 1:200), rabbit monoclonal anti-human AP2α (C83E10; Cell Signaling Technology, 1:100), rabbit polyclonal anti-human NESTIN (Eurofins Genomics, 1:200), rabbit polyclonal anti-human NANOG (ReproCELL, 1:200), and mouse monoclonal anti-human OCT3/4 (C-10; Santa Cruz, 1:200).

    Techniques: Marker, Derivative Assay, Immunocytochemistry, Cytometry